منابع مشابه
Chimeric cardiac troponin I antibodies
most assay manufacturers take additional measures during the assay development. The most frequent procedure involves suppressing the non-specific binding with blocking reagents, which vary from normal mouse IgG to more refined formulations. Whilst in most cases these measures are adequate, they do result in additional costs in terms of both material and labor. The effect of HAMA can also be sup...
متن کاملDegradation of cardiac troponin I in serum complicates comparisons of cardiac troponin I assays.
BACKGROUND Up to a 20-fold variation in serum cardiac troponin I (cTnI) concentration may be observed for a given patient sample with different analytical methods. Because more limited variation is seen for control materials and for purified cTnI, we explored the possibility that cTnI was present in altered forms in serum. METHODS We used four recombinantly engineered cTnI fragments to study ...
متن کاملHydrodynamic properties of bovine cardiac troponin-I and troponin-T.
Bovine cardiac troponin-I (TN-I) and troponin-T (TN-T) have been examined in solution using ultracentrifugation, gel filtration, and viscosity. A new method of purifying TN-T, employing hydroxylapatite chromatography in 6 M urea, is reported. Cardiac TN-T (Mr = 36,000) undergoes a reversible, concentration-dependent association in nondenaturing buffers, probably to a tetramer. The Stokes radius...
متن کاملMyofilament calcium sensitivity and cardiac disease: insights from troponin I isoforms and mutants.
The heightened Ca2+ sensitivity of force found with hypertrophic cardiomyopathy (HCM)-associated mutant cardiac troponin I (cTnIR145G; R146G in rodents) has been postulated to be an underlying cause of hypertrophic growth and premature sudden death in humans and in animal models of the disease. Expression of slow skeletal TnI (ssTnI), a TnI isoform naturally expressed in developing heart, also ...
متن کاملCardiac Troponin I elevation after epileptic seizure
BACKGROUND Cardiac troponin-I (cTNI) is highly specific biomarker to prove myocardial damage, e.g. in acute coronary syndrome (ACS). However, it occurs in other conditions as well. We therefore analysed cTNI increase in patients after generalized convulsive seizure. METHODS Consecutive patients admitted with acute generalized convulsive seizure were included in case of cTNI measurement on adm...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1995
ISSN: 0021-9258
DOI: 10.1074/jbc.270.43.25445